Fig. 3

Highly efficient gene targeting in the absence of Pol θ. a Map of the Rad54 locus showing regions of homology with the gene targeting constructs used in the study. The oval on the construct map indicates the location of the cassette containing the downstream part of the Rad54 CDS fused to GFP, and a selection cassette. b FACS frequency histograms from the Rad54-GFP gene targeting assay in unirradiated and irradiated (200 mGy) cells. Marker used to determine the percentage of Rad54-GFP-positive cells is shown. As the shoulders of RI and GT peaks overlap, the number of events within the marker under-reports or over-reports the true fraction of targeted cells, when the distribution is strongly skewed toward GT or RI, respectively. c Gene targeting efficiency at the Pim1 locus (means and s.e.m. are plotted). d Gene targeting efficiency of exon 9 and exon 18 Rad54-GFP knock-in constructs in wild type (filled histograms) and Polq ^−/− (red outline) cells