Fig. 4

HR frequency and pharmacological manipulation of HR/RI ratio in Polq ^−/− cells. a DR-GFP reporter constructs were integrated in wild-type and mutant cell lines at the Pim1 locus. Reporter-containing cells were co-lipofected with I-SceI expression construct and RFP expression plasmid. Gene conversion frequency (GFP+) and transfection efficiency (RFP+) was measured by FACS 48 h after transfection. Means and s.e.m. are plotted. b Wild-type (left) and Polq ^−/− cells were electroporated with exon 9 or exon 18 Rad54-GFP gene targeting constructs and 10 µM NU-7441 (red outline) was added to the media immediately after electroporation and removed 24 h later. The HR/RI ratio was determined by FACS