Fig. 1 | Nature Communications

Fig. 1

From: Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles

Fig. 1

Comparison of WGA-X and MDA performance with microbial benchmark strains. a Electrophoresis gel images of WGA-X (orange dots) and MDA (blue dots) products obtained from three bacterial benchmark strains and from no-template negative controls (NTC). b Examples of WGA-X and MDA reaction kinetics, where reaction critical point (Cp) is estimated as the time required to reach the inflection point of the reaction’s exponential phase. c Correlation between reaction Cp and genome recovery from SAGs. d Average, standard deviation, and range of genome recovery from SAGs of the five benchmark strains, where SAGs were selected either at random or based on their lowest Cp values. In c and d, each bacterial strain data set derives from eight randomly selected SAGs and five SAGs with the lowest Cp; each eukaryote data set derives from three SAGs with the lowest Cp. Assemblies of bacterial and eukaryote SAGs were produced from five million and twenty million of 2 × 150 bp reads, respectively

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