Fig. 4

Accumulation of HSPC clusters in the AGM of Drosha mutants. a ECs (CD31⁺CD45⁻), HSPC clusters (CD31⁺Kit⁺CD45⁻), maturing HSPCs (CD31⁺CD45⁺), and mature hematopoietic population (CD31⁻CD45⁺) in E12.0 Ctr or cKO AGMs were analyzed by flow cytometry. For ECs (CD31⁺CD45⁻), maturing HSPCs (CD31⁺CD45⁺) and mature hematopoietic cells (CD31⁻CD45⁺); the fraction (%) of each population among live (DAPI⁻) cells was plotted as Mean ± SEM; p values were generated by unpaired Student’s t test. For HSPC clusters, the fraction (%) of CD31⁺Kit⁺CD45⁻among CD31⁺ cells was plotted. n = Ctr: 18 embryos, cKO: 5 embryos. 3 litters. b Whole-mount IF images of HSPC cluster cells in AGMs from E12.0 Ctr or cKO embryos were shown. Runx1 (green) and CD31 (red). Arrows indicate HSPC clusters. Areas shown with a dotted box in left panels were magnified and presented in right. Scale bars: 50 µm. n = Ctr: 3 embryos, cKO: 2 embryos. 1 litter. NS, not significant