Fig. 2
From: Chronic activation of JNK JAK/STAT and oxidative stress signalling causes the loser cell status
JNK pathway activity restricts the growth of prospective loser cells. a List of known JNK targets or regulators differentially expressed in the indicated genotypes (fold changes with false discovery rate>0.1 are in grey). b–d Activation of the transcriptional reporter of JNK activity (TRE16-dsRED, red) in WT (b), RpS15 +/− (c) or RpS3 +/− wing discs (d). e Quantification of fluorescence intensity from images as in b–d for the indicated genotypes, each dot represents a single wing disc. P values according to a post-hoc Tukey test. For all box and whisker plots the horizontal line represents the median and the upper/lower whiskers indicate the lowest/highest point within 1.5*interquartile range of the lower/higher quartile, respectively. f, g Wing disc harbouring 72-hour-old GFP-negative WT clones within GFP-positive RpS3 +/− tissue with (g) or without (f) overexpression of puc only in the RpS3 +/− cells (achieved using upd3-Gal4 to drive expression specifically in loser RpS3 +/− cells). h, i Wing disc with clonal knockdown of puc (tub>puc-RNAi) and GFP expression (green) stained with anti-cleaved caspase-3, (red), clonal boundaries are indicated by a dotted line. j Quantification of the density of apoptotic cells in the centre of the clone or at the clonal boundary (i.e. within a 2-cell diameter of the clone periphery) for discs of the same genotype as in h; each dot represents a single wing disc. k–m RpS3 +/− wing discs harbouring clones overexpressing GFP alone (k) or with the addition of puc (l) or dIAP1 (m). n Size distributions (in pixels) of clones of the same genotypes as in k, m, P values according to a post-hoc Tukey test. o RpS3 +/− wing disc harbouring GFP-overexpressing clones (same genotype as in k) stained with anti-cleaved caspase-3. Detailed genotypes for each figure panel are listed in Supplementary Table 1. Scale bars, 50 μm throughout all figures
