Fig. 4
From: Evasion of immunosurveillance by genomic alterations of PPARγ/RXRα in bladder cancer
Tumor-intrinsic activation of PPARγ/RXRα is negatively correlated with immune infiltration. a Pathway enrichment analysis of genes differentially expressed in RXRA-S427Y, RXRA-S427F and PPARG overexpressing T24 lines relative to respective controls. Top suppressed pathways are shown. The analysis was based on three biological replicates. b Dot plot showing expression correlation of all genes with the curated immune signature (refer to “Methods”) vs. correlation with PPARG in bladder tumors (n = 385) from TCGA. c Heatmap presenting associations between RXRA mutations and PPARG expression with T-cell markers (top, green label), immune checkpoint molecules (middle, yellow label), and pro-inflammatory factors (bottom, lavender label) in TCGA MIBC (n = 385). d IHC staining of PPARγ and CD8 in two representative human bladder tumor samples from a clinical cohort (n = 23, Eisai cohort). Scale bars: 100 μm. e Summary of the IHC results of Eisai cohort shown in d. Distribution of CD8+ T-cell infiltration in bladder tumors expressing high (scores 2–4) or low (score 1) levels of PPARγ protein. f Whisker plot representing IHC staining of infiltrating CD8+ T cells and PPARγ protein expression of MIBC samples from the bladder cancer meta-dataset (n = 118). No expression, score = 1; High expression, score = 4. The bold lines: median; the boxes: interquartile range (IQR); the upper whiskers: min(max(x), Q_3 + 1.5 * IQR); the lower whiskers: max(min(x), Q_1−1.5 * IQR). Statistical analysis was performed using Kruskal–Wallis test
