Fig. 2 | Nature Communications

Fig. 2

From: A three enzyme system to generate the Strychnos alkaloid scaffold from a central biosynthetic intermediate

Fig. 2

Enzymatic activity of GS1 and GS2 and GO (CYP71D1V1). a Reaction of strictosidine with SGD, GS and GO. b Multiple reaction monitoring (MRM) on a LCMS showing compounds at m/z 355 in enzymatic assays of GS1/2 and GO. Isolation and NMR characterization of the two major compounds indicated that they are (16R)-E-isositsirikine and (16R)-Z-isositsirikine (Supplementary Figs 5–16). c MRM (m/z 323) of an akuammicine standard compared with enzymatic products of GS1/2 and GO. THAS1 is a medium chain alcohol dehydrogenase involved in the heteroyohimbine monoterpene indole alkaloid pathway (Supplementary Fig. 1). EV empty vector

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