Fig. 2

T-cell preincubation with aPC reduces allogenic T-cell reactivity and proliferation. a Splenic T-cells isolated from C57BL/6 wild-type (wt) mice (T) were co-cultured with BALB/c allogenic antigen-presenting cells (AgPC) for 96 h and T-cell reactivity was measured by thymidine incorporation during the final 16 h. Concomitant incubation with aPC (a +aPC, 20 nM, every 12 h) reduces T-cell reactivity. b aPC-preincubation (20 nM, 1 h, 37 °C) of mouse pan T-cells (T(aPC) + AgPC), but not of mouse AgPC (T + AgPC(aPC)), reduces T-cell reactivity in the MLR in comparison to T_reg-depleted T-cells (T-Tr + AgPC) and T-cells (T + AgPC). c Mouse pan T-cells without (T) and with (T(aPC)) aPC-preincubation were labelled with eFluor450 and then stimulated with plate bound αCD3 (1 µg/ml) and αCD28 (0.5 µg/ml) antibodies and proliferation was measured on day 1 (D1) and day 6 (D6) by FACS. Results of at least three independent experiments, each containing cells from three different mice a, b are shown; representative histogram plots c from triplicate wells of three independent experiments are shown. Mean value ± SEM a, b; *P < 0.05, **P < 0.01 (a t-test, b ANOVA)