Fig. 3

Chro promotes Grh expression during NSC reactivation. a A diagram of in vivo profiling of Dam-control and Dam-Chro in NSCs. b Quantification of fold enrichment in ChIP-qPCR assay using anti-Chro and control (anti-IgG) based on various predicted Chro-binding sites. The CDS region of Vsx1 is used as control. Error bars indicate ± SD. c Control, chro RNAi, chro ⁻, chro ^612/Df and east RNAi third-instar larval brains were labelled with Grh and Mira. The right panels are enlarged views of regions in the yellow boxes. d NSC clones from control and Mtor ^k03905 were labelled with Grh, Mira and CD8. NSC clones were outlined by white dotted lines. e Mira and Dpn were labelled in control, grh ³⁷⁰ and grh ³⁷⁰/Df(2R)PcLB (grh ^370/Df) NSCs. NSCs with cellular extensions, control, 0%, n = 616; grh ³⁷⁰, 5%, n = 426; grh ^370/Df, 11%, n = 672. f Larval brains from control and grh ^370/Df were labelled with EdU and Mira. Yellow arrows indicated Mira⁺ EdU⁻ NSCs in grh ^370/Df. The central brain is to the left of the white dotted line in c and f. White arrows indicated NSCs in c, d and e, and arrowheads indicated the cellular extension of NSCs in c and e. Scale bars, 10 µm