Fig. 4

GLIM assay of embryo viability. a Bovine embryos were observed under regulated atmospheric conditions, with a 40×/0.75 NA objective and a fully open condenser. As outlined in “Methods”, |γ(r _⊥,t)|_ac is a high-passed version of |γ|, such that |γ(k _⊥,t)|_ac = |γ(k _⊥,t)|F(k _⊥) with F(k _⊥) the filter function defined in Eq. (11). b The internal structure dynamics of embryos was quantified by using DIM, based on the time derivative of the stack. DIM shows a continuous decrease over several hours prior to the embryo’s exhaustion. The raw DIM profiles (black dotted) and their fitted profiles (red)