Fig. 1
From: Living GenoChemetics by hyphenating synthetic biology and synthetic chemistry in vivo
Biosynthetic halogenation enabling Suzuki–Miyaura cross-coupling. a in vivo generation of Cl-pacidamycin, by the natural producer, transformed with a halogenase, followed by in vitro cross-coupling of the compound as a component of cell free extract, at 80 °C (in vivo: in vitro)8, b in vitro generation of reactive bromo-aromatics by RebH variant enzymes, followed by in vitro cross-coupling of the compound as a component of the crude extract (in vitro: in vitro)11, c in vitro generation of reactive bromo-aromatics by stabilized crosslinked enzyme aggregates ((CLEAs) of a series of flavin-dependent halogenases, membrane partitioned from anaerobic, palladium-mediated cross-coupling conditions (in vitro: in vitro)12, d in vivo incorporation of reactive, synthetic iodophenylalanine into peptides, followed by protein purification and cross-coupling of the iodinated protein (in vivo: in vitro)13, e in vivo Suzuki–Miyaura modification of synthetically generated, reactive triflate fluoran (synthetic: in vitro)14, f in vivo generation of reactive 7-Br-tryptophan 2 by engineered E. coli RG-1500 synchronous with the in-culture Suzuki–Miyaura cross-coupling of this reactive metabolite (in vivo: in vivo); and g generation of reactive Br-pacidamycin D 3 by engineered Streptomyces coelicolor RG-1104 concomitant with the in-culture Suzuki–Miyaura cross-coupling of this reactive metabolite (in vivo: in vivo)
