Fig. 4
From: Living GenoChemetics by hyphenating synthetic biology and synthetic chemistry in vivo
Mild cross-coupling of Br-tryptophans and tripeptides. a Mild cross-coupling of unprotected halo-tryptophans at 45 °C using L2-Pd, aconversion is determined by 1H NMR of the crude reaction, bisolated yield after purification by reversed-phase chromatography. b synthetic tripeptides incorporating 7-Br-tryptophan 2 used for cross-coupling studies, c Suzuki–Miyaura cross-coupling products derived from the corresponding tripeptides containing 7-Br-tryptophan. A mixture of Br-tryptophan or bromo-tripeptide, L2-Pd (5 mol%) catalyst, p-Tol-B(OH)2 (3 equiv.) and K2CO3 (6 equiv.) in water-EtOH (4:1) or water was stirred at 45 °C for 48 h. Isolated yields are reported after purification by reversed-phase chromatography
