Fig. 1 | Nature Communications

Fig. 1

From: AGC family kinase 1 participates in trogocytosis but not in phagocytosis in Entamoeba histolytica

Fig. 1

Identification of PtdIns(3,4,5)P3-binding proteins. a Proteins eluted from PtdIns(3,4,5)P3, PtdIns(4,5)P2, PtdIns3P and lipid-free control beads were resolved on SDS-PAGE gel followed by silver staining. b Lipid overlay assay with HA-tagged proteins expressed in E. histolytica trophozoites. c Multiple sequence alignment with various PKC isoforms from human and protein kinase from D. discoideum showing important conserved phosphorylation sites in EhAGCK1 and EhAGCK2 activation loop and hydrophobic motif (marked by black arrow). The conserved phosphorylation site is absent in the turn motif of both proteins (marked by red arrow)

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