Fig. 2

Lnc-LFAR1 is over-expressed in HSCs during liver fibrogenesis. a RNA was extracted from the nuclei or cytoplasm of primary HSCs isolated from normal and fibrotic livers. A total of 1 μg of RNA was used for the qRT-PCR analysis of lnc-LFAR1, lnc-MALAT1 (nuclear retained), and Actin mRNAs (cytoplasm retained). b HCs, HSCs, and Kupffer cells were isolated from livers of normal male Balb/c mice, and relative lnc-LFAR1 expression compared to HSCs was analyzed by qRT-PCR. c Primary HSCs were isolated from livers of Balb/c mice treated for 6 weeks with CCl₄ or oil, and the expression of lnc-LFAR1 and α-SMA was determined by qRT-PCR. d α-SMA and lnc-LFAR1 levels were measured by qRT-PCR in HSCs after culture-induced activation. e, f Primary HSCs cultured at day 3 e and day 12 f were stimulated with TGFβ for 24 h and the expression of lnc-LFAR1 was determined by qRT-PCR. The number of biological replicates for each experiment was n ⩾ 3. Data are presented as means ± s.e.m. P values were analyzed by Student’s t-test. *P < 0.05, **P < 0.01