Fig. 8
From: The liver-enriched lnc-LFAR1 promotes liver fibrosis by activating TGFβ and Notch pathways
Lnc-LFAR1 interacts with Smad2/3 to regulate target genes expression. a qRT-PCR detection of lnc-LFAR1, lncRNA-ENSMUST00000154817 and actin retrieved by Smad2/3-specific antibody compared with IgG in the RIP assay within the single-cell suspensions isolated from mouse liver. b The protein level of α-SMA in lnc-LFAR1 over-expressed primary HSCs simultaneously infected with leni-shSmad2 or lenti-shSmad3 virus was determined by western blot. GAPDH was used as an internal control. Uncropped blots of this figure accompanied by the location of molecular weight markers are shown in Supplementary Fig. 18. c Primary HSCs were infected with lenti-lnc-LFAR1 or lenti-control, and ChIP analyses were performed on indicated genes promoter regions using anti-Smad2/3 antibody. Enrichment was shown relative to input. d Schematic representation of the TGFβ/Smad2/3/lnc-LFAR1 pathway and its function in the progression of liver fibrosis. In a and c, the number of biological replicates for each experiment was n ⩾ 3. Data are presented as means ± s.e.m. P values were analyzed by Student’s t-test. *P < 0.05. *P < 0.05 vs IgG RIP in a; and *P < 0.05 vs LV-control in c
