Fig. 6
Facilitatory effect of endogenous ACh-release on the DG population spikes is augmented by Y1 receptor blockage. a Schematic of conditional hM3Dq viral injection into the medial septum of Chat-Cre driver mice. b A representative microscopic image shows the expression of the virally expressed mCherry tag in cholinergic terminals throughout the dentate gyrus. Scale bar, 25 μm. c The hippocampal cholinergic terminals expressing hM3Dq are activated by perfusion of hippocampal slices with CNO. Total of 10 µM CNO results in a moderate increase of the DG population spike area, which is further augmented by 1 µM BIBP3226 (n = 8 slices). For statistical analysis the last 10 min of each condition are averaged and compared, to ensure full availability or the bath-applied drugs. d Summary graph illustrating the change in average population spike area after CNO and CNO + BIBP3226 application relative to aCSF. e By contrast, BIBP3226 application in the absence of CNO does not affect the population spike area (n = 16 slices). f Comparison of normalized BIBP3226 effects on population spike area shows a significant change only after CNO application (n = 16 slices without CNO and n = 8 slices with CNO). Values are means ± s.e.m. Statistical analysis was done with Fisher’s LSD following one-way repeated measures ANOVA (^^P < 0.01: CNO vs. CNO + BIBP3226; **P < 0.01; CNO or CNO + BIBP3226 vs. aCSF condition) in c and d, paired t-test in e and Student’s unpaired t-test in f (*P < 0.05)
