Fig. 1

Microarray-based profiling of the ncRNA landscape in normal hematopoiesis. a Blood cell populations purified by multicolor flow cytometry from different healthy individuals: hematopoietic stem cells (HSCs), common myeloid progenitor cells (CMPs), granulocyte-monocyte progenitor cells (GMPs), megakaryocytes (MEGA), erythroid precursors (ERY), granulocytes (GRAN), monocytes (MONO), CD4 and CD8 T-cells, NK cells (NKC), and B-cells. Cytospins were prepared after sorting. For HSCs, CMPs, and GMPs representative cytospins from in vitro expanded CB HSPCs are depicted. b Annotation, distribution, and functional classes of the Arraystar Human lncRNA Microarray V2.0 probes according to the indicated databases. c Overlapping features between the Arraystar Human lncRNA Microarray V2.0 and the NCode Human Long Non-coding RNA microarray. d Annotation, distribution, and functional classes of the NCode Human Long Non-coding RNA microarray probes. e Box plots of log₂-probe intensities for mRNAs (n = 978,840), ncRNAs (n = 784,530), and lincRNAs (n = 90,855) from all NCode Human Long Non-coding RNA microarrays. P-values were calculated using the two-tailed Welsh’s t-test