Fig. 3

LINC00173 is a granulocyte-specific lincRNA. a Averaged expression (top) and heatmap of granulocyte fingerprint ncRNAs (top 30 without pseudogenes) which show increasing expression from HSCs to CMPs to GMPs. b RNA-seq of human myelopoiesis: PCA on the 1373 most variable ncRNAs in the data set. The arrow indicates the main trajectory of myeloid maturation. Bl/PM blasts/promyelocytes, MM metamyelocytes, PMN polymorphonuclear neutrophils. c SOM representation of RNA-seq data set revealing three spots of co-regulated metagenes (modules), whose expression properties are depicted in the bar charts below. d–f LINC00173 expression normalized to granulocytes as measured by d the Arraystar Human lncRNA Microarray V2.0 (n = 3–5 per data point), e qRT-PCR (n = 3), and f RNA-Seq (n = 2–4). Error bars indicate ± s.e.m. g The LINC00173 gene locus depicting the array probe and alternative isoforms (according to ENSEMBL GRCh38.p5), together with UCSC genome browser tracks (http://genome.ucsc.edu ; assembly: GRCh38/hg38) of RNA-Seq and ChIP-seq data (BLUEPRINT)²⁴, CAGE-Seq Signals (FANTOM5)²⁵, and sequence conservation (GERP-elements)²⁶ in mature human neutrophils. h Guilt-by-association results for LINC00173. Circle size corresponds to the size of the gene set, and connecting line thickness represents the degree of similarity between two gene sets. Red and blue nodes indicate positive and negative correlation to LINC00173 expression, respectively