Fig. 5

ncRNAs of the DLK1-DIO3 locus control human megakaryopoiesis. a t-SNE of all samples using 240 variance-filtered miRNAs from the NCode Human miRNA Microarray V3 platform. b Heat map of 174 cell-type-specific fingerprint miRNAs. c, d Genome-wide view of log₂-FC for c miRNAs (NCode Human miRNA Microarray V3) and d ncRNAs (NCode Human Long Non-coding RNA microarray) in megakaryocytes compared to all other cell types. Red dots: log₂-FC ≥ 3. The heatmaps show qRT-PCR validation results of z-score transformed 2^ΔΔCt values. e Schematic of the imprinted DLK1-DIO3 locus on human chromosome 14. Below are UCSC genome browser tracks (GRCh38/hg38) of ChIP-seq and RNA-Seq data in CD41⁺ megakaryocytic cells²⁴. f Lentiviral expression of miR-770, miR-136, miR-379, and miR-410 (n = 4) and g RNAi (shRNA)-mediated knockdown of DLK1, MEG3, MEG8, and MEG9 (n = 5) in CD34⁺ HSPCs in vitro. Both plots show the percentage of CD41⁺/CD42b⁺ cells. f, g Data are presented as mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns not significant; P-values were calculated using one-way ANOVA with Dunnett’s post hoc test