Fig. 3

Schematic diagram for visualizing the dynamics of the trimeric CmeB pump incorporated in proteoliposomes using total internal reflection sm-FRET imaging. a The three subunits of CmeB embedded in the lipid vesicle are colored differently. The AF546 and AF647 dyes are in green and magenta, respectively. b Inter-subunit distances measured between the two K843 residues of the form I CmeB trimer. c Inter-subunit distances measured between the two K843 residues of the form II CmeB trimer. d The predicted FRET trajectory and e predicted density plot of the transport dynamics of the CmeB pump if this pump functions via the proposed rotating mechanism