Fig. 4

Dynamics in the apo and substrate bound CmeB pump. a Experimental trace of CmeB single-molecule dynamics with donor (green) and acceptor (red) fluorescence (in the absence of Tdc, top panel; in the presence of 1 μM Tdc, middle panel; in the presence of 10 μM Tdc, bottom panel). b FRET trajectories (blue) and idealization of these FRET efficiencies (red) (in the absence of Tdc, top panel; in the presence of 1 μM Tdc, middle panel; in the presence of 10 μM Tdc, bottom panel). c FRET efficiency population histograms of CmeB (in the absence of Tdc (n = 69 traces), top panel; in the presence of 1 μM Tdc (n = 77 traces), middle panel; in the presence of 10 μM Tdc (n = 73 traces), bottom panel). The histograms were fitted with Gaussian functions. d Transition density plots for the CmeB efflux pump (in the absence of Tdc, top panel; in the presence of 1 μM Tdc, middle panel; in the presence of 10 μM Tdc, bottom panel)