Table 1 Steady-state kinetic parameters for ATP-PRT inhibition in the presence of TIH
From: Uncoupling conformational states from activity in an allosteric enzyme
[TIH] (mM) | ||||
|---|---|---|---|---|
Parameters | 0 | 0.25 | 0.8 | 4 |
V max (s−1) | 0.21 ± 0.01 | 0.28 ± 0.01 | 0.38 ± 0.01 | 0.76 ± 0.01 |
K m,ATP (mM) | 0.23 ± 0.03 | 0.20 ± 0.02 | 0.18 ± 0.02 | 0.16 ± 0.02 |
K i,ATP (mM) | 20 ± 1 | 25 ± 1 | 41 ± 2 | 89 ± 5 |
