Fig. 1
IAA94 inhibits NLRP3 inflammasome activation. a Immunoblot analysis of IL-1β and cleaved caspase-1 (p20) in culture supernatants (SN) of LPS-primed BMDMs treated with various doses (above lanes) of IAA94 and then left stimulated with nigericin, and immunoblot analysis of the precursors of IL-1β (pro-IL-1β) and caspase-1 (pro-caspase-1) in lysates of those cells (Input). b, c ELISA of IL-1β b and TNF c in supernatants from LPS-primed BMDMs treated with various doses of IAA94 and then stimulated with nigericin. d, e Immunoblot d or ELISA e analysis of IL-1β and cleaved caspase-1 (p20) in culture supernatants of LPS-primed BMDMs treated with of IAA94 (150 μM) and then left stimulated with MSU, nigericin, or ATP. f Immunoblot analysis of IL-1β and cleaved caspase-1 (p20) in supernatants from PMA-differentiated THP-1 cells treated with various doses (above lanes) of IAA94 and then left stimulated with nigericin. Data are from three independent experiments with biological duplicates in each (b, c, e; mean ± SEM of n = 6) or are representative of at least three independent experiments a, d, f. Two-way ANOVA b Student’s t-test e **P < 0.01, ***P < 0.001
