Fig. 2
Inhibition of Clics suppresses NLRP3 inflammasome activation. a ELISA of IL-1β in culture supernatants of LPS-primed BMDMs transfected with siRNA against Clic1, 4, or 5 and left stimulated with nigericin. b ELISA of IL-1β in culture supernatants of LPS-primed Clic1 –/–, Clic4 –/–, or Clic5 –/– BMDMs stimulated with nigericin. c ELISA analysis of IL-1β in culture supernatants of LPS-primed wildtype or Clic4 –/– BMDMs transfected with siRNA against Clic1, Clic5, or both of them and left stimulated with nigericin. d, e ELISA d or Immunoblot e analysis of IL-1β in culture supernatants of LPS-primed wildtype or Clic4 –/– BMDMs transfected with siRNA against Clic1 and Clic5, and left stimulated with nigericin, MSU, or ATP. f ELISA of IL-1β in culture supernatants of LPS-primed wildtype or Clic1 –/– BMDMs transfected with siRNA against Clic4 and Clic5, and left stimulated with nigericin, MSU, or ATP. g ELISA of IL-1β in culture supernatants of LPS-primed wild type or Clic5 –/– BMDMs transfected with siRNA against Clic1 and Clic4 and left stimulated with nigericin, MSU, or ATP. Data are from three independent experiments with biological duplicates in each (a–d, f, g; mean ± SEM of n = 6) or are representative of three independent experiments e. Student’s t-test a-d, f, g, **P < 0.01, ***P < 0.001
