Fig. 3
Clics are important for NLRP3 inflammasome activation in vivo. a, b FACS analysis of neutrophil numbers a or ELISA b of IL-1β in the peritoneal cavity of C57BL/6J mice intraperitoneally injected with MSU (1 mg per mouse) with or without IAA94 (50 mg/kg of body weight). n = 6 or 7 per group. c qPCR analysis of Clic5 expression in peritoneal macrophages from Clic4 –/– mice intraperitoneally injected with nanoparticle-encapsulated siRNA (40 μg per mouse). d, e FACS analysis of neutrophil numbers d or ELISA e of IL-1β in the peritoneal cavity of Clic4 –/– mice intraperitoneally injected with nanoparticle-encapsulated siRNA (40 μg per mouse) and MSU (1 mg per mouse). n = 5–7 per group. Data are shown as mean ± SEM and are representative of two independent experiments. Student’s t-test, **P < 0.01, ***P < 0.001
