Fig. 4
Clics-dependent chloride efflux during NLRP3 inflammasome activation. a–c Qualification of the decrease of intracellular chloride in wild type or Nlrp3 –/– BMDMs at different time points after nigericin a, ATP b, or poly A:T c treatment. d, e Qualification of the decrease of intracellular chloride in BMDMs at different time points after nigericin d or ATP e treatment with or without the pretreatment with IAA94 (150 μM). f, g Qualification of the decrease of intracellular chloride in Clic4 –/– BMDMs transfected siRNA against Clic1 and Clic5 at different time points after nigericin f or ATP g treatment. Data are from three independent experiments with biological duplicates in each (mean ± SEM of n = 6). Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001
