Fig. 7
ROS promotes NLRP3 agonists-induced Clics translocation and chloride efflux. a Immunoblot analysis of the indicated proteins in total lysates (Input) or isolated plasma membrane of LPS-primed BMDMs stimulated with nigericin at different time points. b, c Immunoblot analysis of the indicated proteins in total lysates (Input) or isolated plasma membrane of LPS-primed BMDMs stimulated with ATP b or nigericin c for 15 min with or without the presence of MnTBAP (10 μM). d, e Confocal microscopy analysis d or qualification of cells with membrane translocation of Clic4 e in BMDMs stimulated with ATP, nigericin or poly A:T for 15 min. Scale bar, 10 μm. f, g Confocal microscopy analysis f or qualification of cells with membrane translocation of Clic4 g in BMDMs stimulated with ATP or nigericin for 15 min with or without the presence of MnTBAP (10 μM). Scale bar, 10 μm. h, i Qualification of the decrease of intracellular chloride of BMDMs stimulated with ATP h or nigericin i with or without the presence of MnTBAP (10 μM). Data are from three independent experiments with biological duplicates in each (h, i; mean ± SEM of n = 6) or are representative of three independent experiments a–d, f. Student’s t-test g–i, *P < 0.05, **P < 0.01, ***P < 0.001
