Fig. 8 | Nature Communications

Fig. 8

From: CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation

Fig. 8

Clics are important for NLRP3 inflammasome assembly. a Immunoblot analysis of ASC oligomerization in lysates of LPS-primed Clic4 –/– BMDMs transfected with siRNA against Clic1 and Clic5 and left stimulated with ATP or nigericin. b Immunoprecipitation (IP) and immunoblot analysis of the interaction of endogenous NLRP3 and ASC in LPS-primed Clic4 –/– BMDMs transfected with siRNA against Clic1 and Clic5, and left stimulated with ATP or nigericin. c IP and immunoblot analysis of the interaction of endogenous NLRP3 and NEK7 in LPS-primed Clic4 –/– BMDMs transfected with siRNA against Clic1 and Clic5, and left stimulated with ATP or nigericin. d Model for the function and mechanism of Clics and chloride efflux in NLRP3 inflammasome activation. Data are representative of three independent experiments a–c

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