Figure 7
From: Crystal structure of a Pseudomonas malonate decarboxylase holoenzyme hetero-tetramer
Impact of mutations in MDC holoenzyme subunits on growth with malonate as the sole carbon source. Individual deletion of genes for the MdcA, MdcC, or MdcE subunit in P. aeruginosa abolished growth on malonate. Insertion of the coding sequence for the MdcC subunit at the mdcC deletion site restored growth. Deletion of MdcA residues 519–554 (required for MDC hetero-tetramer formation) and the R314E mutation in MdcA (involved in recognition of malonate in the active site) also abolished growth. Finally, Escherichia coli, which is not able to grow on malonate as a sole carbon source, served as a negative control. Attempts at deleting the MdcD subunit were not successful. Results from one representative experiment from three biological replicates are shown
