Fig. 8

PASE EMT phenotype requires SNAI1. a Representative confocal micrographs showing a stage III PASE stained for SNAIL (red) and WGA (purple). HOECHST (blue) counterstains nuclei. n = 2 independent experiments. Scale bars, 50 µm. b Confocal micrographs showing GFP expression and BRA staining in a representative PASE generated from control (wild type) cells (upper; transfected with piggyBac-SpCas9-T2A-GFP vector only) and SNAI1-KO cells (lower; transfected with piggyBac-SpCas9-T2A-GFP-hSNAI1). HOECHST counterstains nuclei. Scale bar, 50 µm. c Quantitated percentages of stage III PASE among all PASE when using an untransfected H9 line, two separate H9 lines transfected with piggyBac-SpCas9-T2A-GFP vector only (Control #1 and #2), and three separate SNAI1-KO lines, respectively. Data represent the mean ± s.e.m, with n = 3–4 biological replicates indicated by individual dots under each condition. n = 3 independent experiments. P-values were calculated using unpaired, two-sided Student’s t-test. ***P < 0.001