Fig. 9
From: A pluripotent stem cell-based model for post-implantation human amniotic sac development
Self-patterned BMP-SMAD signal transduction during PASE development. a Representative confocal micrographs showing a pre-PS PASE on day 5 stained for pSMAD1/5 (green) and WGA (red). HOECHST (blue) counterstains nuclei. n = 2 independent experiments. Scale bar, 50 µm. b Representative confocal micrographs showing stage-wise spatial patterns of pSMAD1/5 (red) and CDX2 (green) in PASE on day 5. HOECHST (blue) counterstains nuclei. Arrow-heads mark pSMAD1/5 + cells in the embryonic disc of the PASE. n = 2 independent experiments. Scale bars, 50 µm. c Cartoon summarizing spatial patterns of BMP-SMAD signaling in the PASE along the amniotic ectoderm-epiblast axis as well as along the medial-lateral axis of the embryonic disc. Color scale represents the intensity of BMP-SMAD signaling ranging from low to medium and high as indicated
