Fig. 3
From: Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancer
Imaging and data metrics of single SK-BR-3 nuclei and cells. a Nuclear suspensions were stained with DAPI (blue) and cytotracker (red), while cellular suspensions were stained with Hoechst (blue) and cytotracker (red) for fluorescent microscopic imaging at ×40 magnification to visualize the nucleus and cytoplasm. The 100 μm scale bar indicates the cell size as a reference. b Nanodispensed nuclei and cells were imaged in nanowells using DAPI, or Hoechst and PI c Nanogrid maps of cells or nuclei that were selected for RNA-seq. d Sequence data metrics were calculated for single nuclei and single cells from the SK-BR-3 breast cancer cell line, where the boxes indicated the 75% interquartile range and whiskers showed the default values of the boxplot, with N = 485 for single nuclei and N = 424 for whole cells
