Fig. 3
From: Retrograde BDNF to TrkB signaling promotes synapse elimination in the developing cerebellum
BDNF requirement for the elimination of CF synapses from the PC soma. a Triple fluorescent labeling for calbindin (blue), VGluT2 (green), and mOrange-positive BDNF-KD PCs (magenta) from a P21 mouse. Asterisks indicate the somata of mOrange-positive BDNF-KD PCs. White and black arrowheads indicate the most diatal tip of VGluT2-positive CF terminals along dendrites of control and BDNF-KD PCs, respectively. b, c Summary bar graphs showing the number of VGluT2-positive terminals per 10 μm membrane of the PC soma (b) and the number of VGluT2-positive terminals per 100 μm2 of the PC somatic area (c). Data were collected from mOrange-nagative control PCs (n = 283) and mOrange-positive BDNF-KD PCs (n = 241) from 3 mice (for b, control: 0.27 ± 0.02, BDNF-PC-KD: 0.44 ± 0.03, P < 0.0001; for c, control: 0.35 ± 0.03, BDNF-PC-KD: 0.87 ± 0.06, P < 0.0001, Mann–Whitney U-test). d Summary bar graph showing the relative height of CF terminals to the thickness of the ML for mOrange-negative control PCs (n = 108) and mOrange-positive BDNF-KD PCs (n = 108), from 3 mice (control: 68.9 ± 0.8, BDNF-PC-KD: 70.4 ± 0.9, P = 0.166, Mann–Whitney U-test). The distance between the most distal tip of VGluT2-posotive CF terminals along dendrites and the center of the soma was measured for each PC and the value was divided by the thickness of the ML. Values in b, c and d represent mean ± s.e.m. ns indicates no significant difference between the two groups. Scale bars, 20 μm
