Fig. 7
From: Retrograde BDNF to TrkB signaling promotes synapse elimination in the developing cerebellum
BDNF and mGlu1 share a common signaling pathway for CF synapse elimination. a Representative CF-EPSC traces for control and mGlu1-PC-KD (from a P24 wild-type mouse) and for BDNF-PC-KO and mGlu1-PC-KD/BDNF-PC-KO (from a P24 BDNF-PC-KO mouse). Holding potential was −10 mV. Single traces recorded at different stimulus intensities were superimposed. b Frequency distribution histogram for the number of CFs innervating each PC showing the effects of mGlu1-knockdown in PCs of wild-type mice (Control, n = 32; mGlu1-PC-KD, n = 32, from 4 wild-type mice during P21–P35, P = 0.018, Mann–Whitney U-test) and in PCs of BDNF-PC-KO mice (BDNF-PC-KO, n = 40; mGlu1-PC-KD/BDNF-PC-KO, n = 37, from 4 BDNF-PC-KO mice during P21–P35, P = 0.419, Mann–Whitney U-test). c, d Similar to a, b but for the effects of P/Q-VDCC knockdown in PCs of wild-type mice (Control, n = 32 PCs; P/Q-PC-KD, n = 33 PCs, from 5 wild-type mice during P21–P35, P = 3.0 × 10−4, Mann–Whitney U-test) and in PCs of BDNF-PC-KO mice (BDNF-PC-KO, n = 40 PCs; P/Q-PC-KD/BDNF-PC-KO, n = 33 PCs, from 4 BDNF-PC-KO mice during P21–P35, P < 0.0001, Mann–Whitney U-test). CF-EPSCs in c were from a P35 wild-type mouse (Control and P/Q-PC-KD) and from a P36 BDNF-PC-KO mouse (BDNF-PC-KO and P/Q-PC-KD/BDNF-PC-KO). e, f Similar to a, b and c, d but for the effects of GluD2 knockdown in PCs of wild-type mice (Control, n = 32 PCs; GluD2-PC-KD, n = 34 PCs, from 4 wild-type mice during P21–P35, P = 5.0 × 10−4, Mann–Whitney U-test) and in PCs of BDNF-PC-KO mice (BDNF-PC-KO, n = 40 PCs; GluD2-PC-KD/BDNF-PC-KO, n = 36 PCs, from 4 BDNF-PC-KO mice during P21–P35, P < 0.0001, Mann–Whitney U-test). CF-EPSCs in e were from a P23 wild-type mouse (Control and GluD2-PC-KD) and from a P36 BDNF-PC-KO mouse (BDNF-PC-KO and P/Q-PC-KD/BDNF-PC-KO). Scale bars, a, c, and e, 10 ms and 1 nA. *P < 0.05, ***P < 0.001, ****P < 0.0001. ns indicates no significant difference between the groups
