Table 2 Ratios of peak areas reflect the relative concentrations in solution of the measured proteins

From: Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis

Concentration ratio

Peak area ratio

 

E9S84A:E9WT

E9F86A:E9WT

Im2N34A:Im2WT

Im2D33A:Im2WT

1:1

1.03 ± 0.09

0.97 ± 0.03

0.98 ± 0.02

1.02 ± 0.02

2:1

2.05 ± 0.16

2.04 ± 0.07

1.97 ± 0.14

2.01 ± 0.19

1:2

0.49 ± 0.02

0.49 ± 0.04

0.51 ± 0.02

0.52 ± 0.01

  1. The ratios of peak areas of the mutant and wild-type proteins (Fig. 2) are in excellent agreement with their concentration ratios of 1:1, 2:1, and 1:2 in solution
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