Table 4 Values of the coupling energy of Phe86 in E9 with Asp33 in Im2

From: Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis

Ratio

1:2:8:2

1:1:4:1

2:1:4:1

Charge state

+8

+9

+8

+9

+8

+9

ΔΔG int(ij) (kcal mol−1)

1.08 ± 0.10

1.08 ± 0.05

1.28 ± 0.15

1.27 ± 0.20

1.32 ± 0.16

1.21 ± 0.07

  1. Values of the coupling energy, ΔΔG int(ij), were calculated for the charge states +8 and +9 using the concentration ratios 1:2:8:2, 1:1:4:1, and 2:1:4:1 as defined in Fig. 4. Similar coupling energies are obtained for the different concentration ratios
Back to article page