Fig. 3
Long-term NP retention and MHC class II knockdown in HUVECs. a siRNA-loaded nanoparticles (red) are retained in HUVECs for at least 11 days. Cells were stained using phalloidin (green) and dapi (blue) and imaged using a confocal microscope (scale bar: 100 μm). b Dilution of fluorescence over 11 days in HUVECs pre-labeled with PKH67 tracer (Sigma). Labeled HUVECs were seeded in a 24-well plate at a concentration of 105 cells per well. Cell tracer fluorescence was measured using a flow cytometer. c Durable knockdown of HLA-DR protein on HUVECs for 11 days. HUVECs were treated with siRNA-loaded nanoparticles or Lipofectamine for 6 h and cultured for up to 11 days. At days 3, 5, 7, 9, and 11, cells were harvested and surface HLA-DR expression was quantified using a flow cytometer. Data shown is mean ± SD (n = 4). **p < 0.01, ***p < 0.001, ****p < 0.0001 by Student’s t-test
