Fig. 5

NP perfusion reduces CD4 + memory T-cell proliferation. a CD4 + memory T-cell proliferation. Umbilical veins were perfused with culture medium containing siRNA-loaded NPs (0.1 mg mL⁻¹) or CTL siRNA-loaded NPs (0.1 mg mL⁻¹). After 6 h, ECs were harvested from veins and seeded into 24-well plates. CFSE-labeled CD4 + memory T cells were co-cultured with endothelial cells for 7 days. T-cell proliferation was then quantified using flow cytometry b HLA-DRA/GAPDH mRNA was quantified 24 h after addition of hIFN-γ by quantitative reverse transcription-PCR. c, d HLA-DR protein was quantified by flow cytometry. e CD4 + memory T-cell proliferation was determined by CFSE dye dilution on flow cytometry. Data shown is mean ± SD (n = 5), ****p < 0.0001