Fig. 7
NPs reduce intimal expansion and T-cell infiltration in transplanted human arterial allograft. a Human arteries were pretreated with siRNA-loaded nanoparticles for 6 h and transplanted into SCID/bg mice previously reconstituted with 1.5 × 108 allogeneic human PBMCs. After 10 days, arteries were harvested, sectioned, and stained. b CIITA nanoparticle pretreated arteries (n = 4) or CTL nanoparticle pretreated arteries (n = 4) were sectioned and stained using H&E and EVG. Intimal area and vessel wall thickness were quantified using ImageJ (scale bar: 200 μm). c Artery sections were stained for human α-SMA (green), human CD31 (red), and DAPI (blue). α-SMA and endothelial cell coverage was quantified using ImageJ (scale bar: 100 μm). d Artery sections were stained for CD45RO (green) and DAPI (blue). Total number of CD45RO + cells in the intima (per cross-section) was quantified from fluorescent images (scale bar: 100 μm). Data shown is mean ± SD, *p < 0.05, **p < 0.01, by student’s t-test
