Fig. 2
From: Non-coding RNAs participate in the regulatory network of CLDN4 via ceRNA mediated miRNA evasion
CLDN4 reinforces the proliferative and invasive capacity of GC cells in vitro. a Cell proliferation was assessed daily for 4 days using the Cell Counting Kit-8 (CCK-8) assay in CLDN4 overexpressing SGC-7901 cells. b Transwell assays were used to evaluate the involvement of CLDN4 for invasion in CLDN4 overexpressing SGC-7901 cells. c Scrape motility assays were monitored at 0, 6, 12, and 24 h in CLDN4 overexpressing SGC-7901 cells. d Cell proliferation assessed in CLDN4 overexpressing AGS cells. e Transwell assays assessed in CLDN4 overexpressing AGS cells. f Scrape motility assays in CLDN4 overexpressing AGS cells. g Cell proliferation assessed in CLDN4 knockdown SGC-7901 cells. h Transwell assays assessed in CLDN4 knockdown SGC-7901 cells. i Scrape motility assays in CLDN4 knockdown SGC-7901 cells. j Cell proliferation assessed in CLDN4 knockdown AGS cells. k Transwell assays assessed in CLDN4 knockdown AGS cells. l Scrape motility assays in CLDN4 knockdown AGS cells. In b, e, h, and k, cells were incubated for 24 h, and counted under the microscope. Original magnification ×200. Scale bars = 100 μm. Data are shown as mean ± s.d., n = 3. The data statistical significance is assessed by Student’s t-test. *P < 0.05, **P < 0.01
