Fig. 3
From: The non-coding RNA BC1 regulates experience-dependent structural plasticity and learning
BC1 KO pyramidal neurons display increased spontaneous synaptic activity. a Sample traces of spontaneous EPSCs (sEPSC) recorded from layer 2/3 pyramidal neurons of WT and BC1 KO barrel cortex. Scale bars, 20 pA, 100 ms. b–d Quantification of sEPSC amplitude, frequency and kinetics (**P < 0.01, two-tailed t-test, mean ± s.e.m, n = 13 and 17 neurons from WT and KO mice, respectively). Outliers from b and d (closed circles) were excluded from the statistical analysis. n.s., not significant. e Scheme illustrating the stimulation of principal (PW) and surrounding whiskers (SW). f Spontaneous activity in layer 2/3 barrel cortex neurons (*P < 0.05, unpaired t-test with Welch correction, mean ± s.e.m., n = 4 and 10 neurons from WT and KO mice, respectively). g, h Stimulation of PW and SW and response rates of layer 2/3 pyramidal neurons (P = 0.4141 and 0.4201, unpaired t-test with Welch correction, mean ± s.e.m., n = 4 and 10 neurons from 3 WT and 5 KO mice, respectively). i left Photomicrographs of cytochrome oxidase (CO)-stained barrels in somatosensory cortex of WT and BC1 KO mice. Scale bar, 500 µm. right Scatter plot of the mean optical density of CO staining in the barrels. Dots represent individual barrel values (***P = 0.001, Mann–Whitney U-test, mean ± s.e.m., n = 18 barrels)
