Fig. 1 | Nature Communications

Fig. 1

From: Single cardiomyocyte nuclear transcriptomes reveal a lincRNA-regulated de-differentiation and cell cycle stress-response in vivo

Fig. 1

Single nuclear RNA-seq reveals CM heterogeneity. a, b Core cardiac genes that are most highly expressed in every CM nucleus (a) exhibit high expression with low coefficient of variation (b). c Highly expressed genes in TAC nuclei have higher penetrance than highly expressed genes in Sham nuclei. Spearman’s rank correlation (r = 0.90, p < 2.2e−16) shows good correlation between average expression level and penetrance. d Density distribution of correlation shows higher correlation in TAC nuclei than in Sham nuclei. p < 2.2e−16 from Mann–Whitney U test. e, f Unsupervised hierarchical clustering e and PCA f of single nuclear RNA-seq of CM reveal that CM nuclei accurately segregate into clusters specific to Sham or TAC subgroups (subgroup a, b) and is replicated across biological repeats (Rep) f. g Ranked Spearman correlation plot shows higher correlation in TAC nuclei than in Sham nuclei, which is replicated across biological repeats (Rep)

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