Fig. 3 | Nature Communications

Fig. 3

From: Single cardiomyocyte nuclear transcriptomes reveal a lincRNA-regulated de-differentiation and cell cycle stress-response in vivo

Fig. 3

Human single cardiomyocyte nuclear RNA-seq. a Core cardiac genes in human CMs are similar to mouse. b–d Unsupervised hierarchical clustering (b), PCA (c) and Spearman correlation analysis (d) produced 2 distinct subgroups in each of control and dilated cardiomyopathy (DCM) nuclei. e Density distribution of correlation shows narrower distribution for DCM nuclei compared to control. P value from Mann–Whitney U test. f WGCNA identifies gene modules (healthy 1, healthy 2, disease 1, and disease 2) that are specific for DCM or control nuclear subgroups. g, h Classifiers from human gene modules show differential expression at single nuclear level (g), but not in matched bulk left ventricle RNA-seq (h)

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