Fig. 6 | Nature Communications

Fig. 6

From: Single cardiomyocyte nuclear transcriptomes reveal a lincRNA-regulated de-differentiation and cell cycle stress-response in vivo

Fig. 6

Gas5 and Sghrt regulate co-regulated network gene expression. a Strategy to KD Gas5 or Sghrt independently in isolated adult TAC CMs in vitro. Cartoon from openclipart.org, under a CC0 1.0 Universal license. b, c In vitro KD of Gas5 or Sghrt in adult mouse CMs using GapmeRs is efficient and reproducible across biological replicates. N = 5 biological replicates. d–g Gas5 KD in TAC CMs results in significant reduction of Nppa, Dstn, Ccng1, and Ccnd2 expression. Sghrt KD in TAC CMs results in significant increase in Ccng1 and reduction in Ccnd2 expression. N = 5 biological replicates. Data represented as mean ± s.e.m. *p < 0.05, **p < 0.01, and ***p < 0.001

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