Fig. 1
From: Timing of Smarcb1 and Nf2 inactivation determines schwannoma versus rhabdoid tumor development
P0-CreC;Smarcb1 flox/flox mice develop RTs in NC-derived tissues. a Genotype distribution of mice at 3 weeks of age (n = 282) born from crossings of P0-CreC;Smarcb1 flox/+ and Smarcb1 flox/flox mice. b Kaplan–Meier curve representing the percent survival of P0-CreC;Smarcb1 flox/flox (n = 141, median survival = 3.2 months), P0-CreC;Smarcb1 flox/+ (n = 29, median survival = 21.6 months) and Smarcb1 flox/flox (n = 33, median survival > 24 months) mice versus age in months. c T2-weighted MR image identifying a tumor mass in the nasal cavities of a P0-CreC;Smarcb1 flox/flox mouse (arrow). d X-gal staining of a tumor from P0-CreC;Smarcb1 flox/flox;ACZL mice demonstrating activation of the beta-galactosidase reporter gene ACZL (blue staining) thus attesting for Smarcb1 deletion throughout the tumor and in rhabdoid cells (arrow and inset). e Smarcb1 del allele is detected in all the tumors from young P0-CreC;Smarcb1 flox/flox mice (< 10 months of age). Long-term P0-CreC;Smarcb1 flox/flox survivor mice (Supplementary Fig. 2c) develop liver, lung, and uterus tumors, with no deletion of Smarcb1 similar to control littermates (Smarcb1 flox/flox). These tumors are part of the normal tumor spectrum of aging FVB/N mice24. f, g P0-CreC;Smarcb1 flox/flox mice develop tumors along the olfactory nerve (arrow), close to the olfactory bulb (f) or in the nasal cavity below the olfactory epithelium (double arrow, g). h X-gal staining of the nasal cavity of a P0-CreC;AZCL mouse showing specific Cre recombinase expression in SCs of the olfactory nerve (arrow) below the olfactory epithelium layer (double arrow). i Tumor of the trigeminal nerve (transversal section). j X-gal staining of the trigeminal nerve of a P0-CreC;AZCL mouse showing specific Cre recombinase expression in SCs (arrows). k Tumor of the vestibulocochlear nerve (dashed square) invading the cochlea (arrow). Note that the contralateral side is not affected. l Detail of the vestibulocochlear nerve tumor shown in k. m Tumors in the eye (black arrow), optic nerve (white arrow), and oculomotor nerve (striped arrow) of a P0-CreC;Smarcb1 flox/flox mouse. n Tumors in the spinal nerve roots (arrows, longitudinal section). Insets in f, i, l, m and n represent a higher magnification of the dashed square area (insets scale bar is 20 µm). **(P < 0.01) and ***(P < 0.001); log-rank (Mantel–Cox) test
