Fig. 1
From: Poliovirus intrahost evolution is required to overcome tissue-specific innate immune responses
RNA viral evolution capacity is required to overcome the type I interferon (IFN) response. a The structure of RNA dependent RNA polymerase (RdRp) of poliovirus. G64S (G) confers higher replication fidelity and D79H (D) reduces recombination rate24,25,26. b Mutation rates associated of wild type (WT) and G64S/D79H (GD) poliovirus. Mutation rates are shown for G to A, C to U and U to A substitutions, based upon the frequency of lethal mutations (P < 0.05, seven passages, n = 7, mean ± s.d.). c Recombination rates for WT and GD poliovirus. For additional details see Supplementary Fig. 1. d Percentage survival of Tg21 mice or IFNAR−/− mice (n = 10 for each cohort) infected with virus by I.P. inoculation route. Mice were inoculated with 108 plaque-forming units (PFU) per Tg21 mouse or with 104 PFU per IFNAR−/− mouse (n = 10 for each cohort). e 50% lethal dose (LD50) by intra-peritoneal (I.P.) and intra-muscular (I.M.) route (n = 10 for each cohort)
