Fig. 4

Genetic composition of poliovirus promotes intrahost evolution through counteracting tissue specific interferon responses. a Tissue-specific patterns of diversity in organ-resident populations. Virus was isolated at 3 days postinfection in Tg21 mice (n = 3, the number of mice is three for each time point and each group). Liver (LV), kidney (KD), and spleen (SP). Diversity is represented as Shannon’s Entropy. b The genetic structures of in vivo adapted populations. Multidimensional scaling correlation. In vivo adaptation leads to tissue-specific genetic structures (colored ovals) in WT populations from LV, KD and SP, but not in GD populations (grey oval). c Correlation plot of diversity between tissue-specific adapted populations between viral strains. d Model highlighting how efficient recombination and mutation leads to rapid adaptation during intrahost spread. G64S/D79H(GD), engineered to be deficient in recombination and diversity, is limited in its ability to rapidly adapt to changing selection pressures