Fig. 6
From: Poliovirus intrahost evolution is required to overcome tissue-specific innate immune responses
Adaptive 2B mutants increased virus replication and pathogenesis immune-competent mice. a, b The adaptive 2B mutants induce less IFN response in primary murine embryo fibroblast cells (MEFs). a MEFs were infected with WT and 2B mutants at m.o.i=0.1. supernatant was collected to measure IFNβ by ELISA at 72 h postinfection. RNA copy number of poliovirus genome was measured by qRT-PCR on MEFs at postinfection 72 h. b mRNA expression level of IRF7 and ISG56 was measured by qRT-PCR on MEFs at postinfection 72 h (n = 3, three replicates for each time, for each viral strain. Student’s t-test). n.s. indicates P > 0.05; *P ≤ 0.05, **P ≤ 0.01. c Virus tissue distribution. Viral titers in kidney, muscle and brain of Tg21 mice inoculated with 106 PFU of WT, 2B I2V, 2B I6V, and 2B I6M administered I.P. route. Data were presented as logarithm(mean ± s.d.), PFU per ml. n = 4, the number of the mice is four per time point per viral strain. Limited detection level is 20 PFU per gram tissue. d Survival curve of Tg21 mice Tg21 mice were injected with 107 PFU WT, 2B I2V, 2B I6V, or 2B I6M administered by I.P. route. (n = 10, the number of the mice is ten per group)
