Fig. 5
From: Brassinosteroid signaling-dependent root responses to prolonged elevated ambient temperature
Elevated ambient temperature impinges on BRI1 levels and brassinosteroid perception. a Western blot analyses monitoring the accumulation of BRI1 protein in 15-day-old plants expressing a functional BRI1-mCitrine (mCit) fusion protein, under the control of the BRI1 promoter, using anti-GFP antibodies. The asterisk represents a non-specific band used as loading control. A representative blot is shown. b Quantitative RT-PCR monitoring BRI1 gene expression in roots from wild-type plants grown at 21 or 26 °C (mean ± s.d., n = 3). The difference in BRI1 gene expression at 21 and 26 °C is not statistically significant. c Influence of elevated ambient temperature on BRI1-mCit protein accumulation in roots. Similar confocal detection settings were used to compare plants grown at 21 and 26 °C. Representative images are shown. Inset shows higher gain. Scale bar, 50 µm. d Phenotype of 15-day-old wild-type plants grown at 21 or 26 °C and treated with mock or 10 nM brassinolide (BL). Representative images are shown. e Primary root length of plants grown as in d (mean ± s.d., n = 25). The asterisks indicate a statistically significant difference (t-test, P < 0.0001). f Influence of increased growth temperature on BRI1-mCit and its non-ubiquitinatable BRI125KR-mCit variant. Similar confocal detection settings were used to compare the effect of temperature. Representative images are shown. Scale bar, 50 µm. g Ratio of primary root length from wild-type, bri1, bri1/BRI1-mCit, and bri1/BRI125KR-mCit plants grown at 21 and 26 °C for 15 days (mean ± s.d., n = 4). The asterisks indicate a statistically significant difference (Kruskal–Wallis; *P < 0.01; **P < 0.001; ***P < 0.0001)
