Fig. 1

Overview of the eQTL study and transcriptional immune response in primary human monocytes. a Step-wise experimental design to identify genetic effects on immune response in human monocytes. (1) Isolation and stimulation of primary monocytes from 134 individuals, (2) Transcriptome measurement of the entire cohort at two time points (90 min and 6 h) after stimulation, (3) Genotype profiling to map immune response eQTLs. b Mean mRNA profiles of differentially expressed genes (log₂-fold change > 1, FDR 0.001) of 134 individuals between baseline and each of the six stimulated conditions. Genes are hierarchically clustered into six distinct expression patterns (Supplementary Data 1 for a full list of the differential expression and enriched pathways of each cluster)